Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKELSRLVHRSFHIIPRQEDFRPLALGASGRTIVRIHFGGRTCIGIQWGND----RADN-DSFIPAARHLHAHGVNVPEIYDYEPL-----GPGCGAALVEDLGDANLLAFRKEPWPSLRLRYIRAMEQLHLLHSCPFPEEFPLQP-AFDEAL-YRWEQSYFAEHFL----GSHLGLETASFLNHPALKEQA---QFLA--ALPECPVHRDSQSQNVHIHA---GKTWLIDFQGMRGGRPEYDLASLVYDGYA------------RLEPEQADELLREWENISAHSIDHRIFRACALQRIMQMLGAYANIGHNQGKTWYLAQIPAGLGHLRKLLPGSTLAEPLAAMLA 4OCV Chain:A ((29-298)) -----FDVASHFALEGTVDSIEPYGDGHINTTYLVTTD-GPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVP-----NPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVG-------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 939 -20804 -22.15 -88.90 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -22.15 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.192

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: