Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTGHGMAGDDWQYRKTMPQNILRPTVEAALPH-V-----AGAEWEPLAGGRTNPVFKIN-A---------DAPLVCKLSCLSGETPLFGNRALDEAAILKHLEGSGLAPSLTDVLAVPEGEILIYSFVEGDILTQ-------TIPAAVRILRQVHQAEIPDNLPRLDGSSAAIIQKTKAILETLPV--------------QKA----M-AISFAEPKVELPDTLVPRLIHGDPVPANFIQSK-------GKLTLIDWQCPAIGDPCEDLAIALSPAMNAVY----------GDGPLTPQQMKECLNAYGD-RTV-----------------SDRYRKLAPLFHWRMAAYCAWKVEQGD--KDYAAGVKLELAALK 5FTG Chain:A ((8-368)) -----------------TRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG---------EAMVLESVMFAILAERSLGPKLYGIFP---Q-GRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFN-KEPK----WLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRS-LLE-STPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDA--

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 959 -4625 -4.82 -17.99 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -4.82 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: