Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLACGSDDLRTEEGLRIAALYGWDPEDTACQLLQRGENGTFLVR-TSGTRYILRRYRRERYREQEILAELAWMCALR-DHINVPNVIANTEGDLATRLVGPDGERLYAVFEFIEGE-SPESS-SSEDYRRLGELMKALHRGADAIAQSMNEDWAGWNRPVYDVQRAVREPLRHLLQFEALSEVNKTRCVSVARELERRFRTLQPGRPFVHADLHFGNIVVREP-NWYCLDFDECGYGHRAFDLGVVRLHLRNRSDNFDCWAHFEHGYGDMPSRGDLATGTAARIFYMAGKIPMRQDIDSLRHNPDARIGKYLTWIEDELM 4R78 Chain:A ((18-278)) -----GSHMEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTT-NKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDI-------------EAGIKVNEYIESAITLDSTSIKTKFDKIAPILQTIHTSAKELR--GEFA----PFEEIK--KYESL-IEEQ-----IPYANYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESE---FTSQEEETFLSHYESDQTPVSHE---KIAIYKILQDTIWSLWTVYK--------------------

General information: TITO was launched using: RESULT: Template: 4R78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1187 12026 10.13 46.98 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 10.13 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.209

(partial model without unconserved sides chains): PDB file : Tito_4R78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r78-query.scw PDB file : Tito_Scwrl_4R78.pdb: