Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISNTPSFPASWNIEAAKRIADTPAGIVYELTRKD--------GSLAIAKVLKKKVL-----KDSLRGADFIAWRAGIGCVELLDQSDNILLMEHA-GTETLRDVLFRDGNDDATTEIAAEVLLRYHQPSEQSPPSS---LLTLPLYFESLFRKAEQDR---SD---GV-DSPFVEAARLAQTLIDQQRDIKPLHGDLHHENIMHSD-------RGWIIIDPA-GLIGDAALDVANMFSNPLDRFD------------LTRSEARIASMAAIFSRALQR-------------D---ERLLLQYAFAYGCLSATWHEEDGNAEERDNELAVAATIKAVLKHF 5FUT Chain:A ((33-351)) ---------------HISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG----GAEAMVLESVMFAILAE-RSLG-PKLYGIFPQGRLEQFIPSR-RLDTEE-L--SLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKI--------------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 809 4670 5.77 19.79 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 5.77 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.283

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: