Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTHVHFPWYLHSIRKQRRNTCNILQNLVSNSKTSLQNAPVLYLRFLASYRVSSETTASEAICLPTIAEIEAATELISAPDASAKVVRV---NERFAVKMGYG-------IPLIEAENMKFLAANSKVPVPKVHAAFRDPDTNKTYIIMEYLPGHTLEKLLPSLDPVEKATISNLIKDAITELRKGLDPKISGP--FANQ-GDMNLAIIEKIRQ-----TESD---QYIRLLRNMIDRTLNSHRSVFTHGDLQPKNIMVERFGDRNVCPQFRISLLDWESAGWYPELWDFCNATIACRFKPDWLELVPDILEQYPVEFLMMQVVYSSVFY 1L8T Chain:A ((22-247)) -----------------------------------------------------------------------------TEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEG--KLPVPKVLHFERH--DGWSNLLMSEADGVLCSEEYED--EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGK--------VSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIK-------------

General information: TITO was launched using: RESULT: Template: 1L8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 883 32194 36.46 157.04 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 36.46 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.273

(partial model without unconserved sides chains): PDB file : Tito_1L8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1l8t-query.scw PDB file : Tito_Scwrl_1L8T.pdb: