Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLLPNPERAWERAAHVQTLARAEIERRIPPFT----GEVEVLSGGHANVNVRLGAE---RVLRVYRRDASAAPKEASLLAHGWRSFR-VPAVLSAGD----------DFLVLEHVPHVPLQDT-AMCGAAVGRALAEIHGVGYDSAGFLGPELAVSVPFADCIGALRGHVDAVLDRLAPA-------LR-------GELS----E---RVRAFLDAN-AEAL-RALASAPVLLHGDFKASNLHWLSSGELLVLDWEFAYAGPGLMDVGQLLRWSPG------REFVAAFAGAYRESAPLPEEFERWAAAFDLFNLVGLLDGVEPGSRRAADVCGRISEVVER 2Q83 Chain:A ((22-299)) ------------------------LAENVLQGWDVQAEKIDVIQ----ALVWKVHTDSGAVCLKRIHRPEKKALFSIFAQDYLAKKGMNVPGILPNKKGSLYSKHGSFLFVVYDWIEGRPFELTVKQDLEFIMKGLADFHTASVGYQP-PNG-VP---I-FTKLGRWPNHYTKRCKQMETWKLMAEAEKEDPFSQLYLQEIDGFIEDGLRIKDRLLQSTYVPWTEQLKKSPNLCHQDYGTGNTLLGENEQIWVIDLDTVSFDLPIRDLRKMIIPLLDTTGVWDDETFNVMLNAYESRAPLTEEQKQVM-----------------------------------

General information: TITO was launched using: RESULT: Template: 2Q83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 835 19448 23.29 86.05 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 23.29 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.301

(partial model without unconserved sides chains): PDB file : Tito_2Q83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2q83-query.scw PDB file : Tito_Scwrl_2Q83.pdb: