Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEAARETRLPCAAMAILTPLSLADARRIGALYGL-EIADVRGLLAGSVNSNFALTLADGRGQVFLRVYEEQQ-LGAASREARMLEHLAAG-GVATPQPLRRRDAAPSDTDAASDGAAFVAEHAGKPVALFPWVGGASLCQAR---VTPDATRRVGAALARVHLVGASFEGAN-ASRFDLDGLDQRLRGLRSPAGARSTAPAPALPPDVAAAVDELTGRLERIRAAAPRTPGPQTLIHGDLFRDNVLWEA-GEISALLDFESASRGSAAFDLAVTMLAWCFGDDLDPDLVSALAAGYTAVRPLSTEERDRLFHESVIAALRFSITRITDFELRPKGSGVYKDFRRFLARQRTLERLGPEGLLALLGV 5IGI Chain:A ((2-258)) --------------TVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDD-GRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVAN-----------AE-LV-AYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRR----------EFV-VNDKRLHRWQRWLDDDS-SWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVF------GEEGLAKLLLTYEAAGGRV---------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1034 -21757 -21.04 -87.73 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -21.04 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.230

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: