Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSEALEQFVVEEAGVGAPRGASWSRVDEGQGANRVWRVRWGP-GSSVIVKRCGPGRAFVQERRAATRWAPAVADASRGLGHARVLAASQALRCLVFVDA-GRGEEQV-ALSPAAFACAGRYLARLHALPVDERDPVP----------LVEALARREARWLEGAPE------------------LRAVASSLGPGALAD--A-DWGPRVPCHRDFQPDNWLWDG-RTLTVVDFEHARPDARALDLAKLWTRLD-------GDVDARANAAFWSAYLGPEPCS--PSFLAQLRAGLVLHGLASVAWGRSHD----DEAFVAEGERAVALARSGREPPNPRAGTPLASL 2QG7 Chain:D ((110-452)) --------------------SLEFQIIN-GG-TNILIKVKDMSKQAKYLIRLYGPKTD---NREREKKISCILYNKN--IA-KKIYVFFTN--GRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDS-IVEVESLCKRENSPIVLCHCDLLSSNIINTVGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSSDFDFINYGMTRLTASCLP---------------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 979 -7006 -7.16 -27.69 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain D : 0.64 3D Compatibility (PKB) : -7.16 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: