Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLLPNPERAWERAAHVQTLARAEIERRIPPFT-GEVEVLSGGHANVNVRLGAERVLRVYRRDA--SAAPKEASLLAHGWRSF--R-VPAVLSAGDDFLVLEHVPHVPLQDT----------AMCGAAVGRALAEIHGVGYDSAGFLGPELAVSVPFADCIGALRGHVDA---VLDRLAP---AL--RGELSERVRAFLDANAEALRALASAPVLLHGDFKASNLHWLSSGELLVLDWEFAYAGPGLMDVGQLLRWSPG---REFVAAFAGAYRESAPLPEEFERWAAAFDLFNLVGLLDGVEPG--SRR-AADVCGRISEVVER 4DCA Chain:A ((25-312)) -------------------LDAEIYEHLNKQIKINELRYLSSGDDSDTFLCNEQYVVKVPKRDSVRISQKRELELYRFLENCKLSYQIPAVVYQSDRFNIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKELHSIEIDCSVSLFS-----DAL---VNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILSNAV---LFKYTPCLVHNDFSANNMIFRNNRLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGRKVLKYYQHKAPE--VAERKAELNDVYWSIDQIIYGYERKDREMLIKDVSELLQ-----

General information: TITO was launched using: RESULT: Template: 4DCA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1081 -9245 -8.55 -35.83 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -8.55 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_4DCA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4dca-query.scw PDB file : Tito_Scwrl_4DCA.pdb: