Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTHVHFPWYLHSIRKQRRNTCNILQNLVSNSKTSLQNAPVLYLRFLASYRVSSETTASEAICLPTIAEIEAATELISAPDASAKVVRVNERFAVKMGYGI-----PLIEAENMKFLAAN--SKVPVPKVHAAFRDPDTNKTYIIMEYLPGHTLEK-LLPSLDPVEKATISNLIKDAITELRKGLDPKISG-PFANQGDMNLAIIE---KIRQTESDQYIRLLRNMIDRT----LNSHRSVFTHGDLQPKNIMVERFGDRNVCPQFRISLLDWESAGWYPELWDFCNATIACRFKPDWLELVPDILEQYPVEFLMMQVVYSSVFY 5IQI Chain:C ((34-257)) -----------------------------------------------------------------------------IGSGYDSVAYLVNNEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYIS--DELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNR-------LTGIIDFGDSGIIDEYCDFIYLLEDSEEEI-GTNFGEDILRMYGN--------------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 787 -4803 -6.10 -23.09 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.64 3D Compatibility (PKB) : -6.10 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.258

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: