Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPINHEPLFDVLSQYDIKVVSIRNESYKDKKGVWWIQTPDEYKIL-KKISNSEDTFKYILSAAEHLRKNGVNIPAVYKTKDGKDYVNINGTCYVLYEAVEGKNPSYNSP-EDFRAIVRELAGFHAASVGFSPPDNTKPKIHLGKWVEQYTEQVEDMNRFYQTELEKS-ENDRIGKVIIEEFPAFYERAKQAIEGLKGKEYQDWVEKVKSRG-GLCHQDFAAGNLLKNPS----GKIFVLDTDSITIDIPARDIRKLLNKIMKKNGKWDL-EILRKF-IRIYQSENPLSFSEWTVVKFDLMFP-H-LFLGAMNKFYYKRDKEWSFEKYLKRINEMTAL--EKTITPVLENFDSIVYEEINQRKD---- 3CSV Chain:A ((4-333)) --SREDEIRDFLATHGY------ADWNRTPRYQRLRSPTGAKAVLMDWSPEEGGDTQPFVDLAQYLRNLDISAPEIYAEEHARGLLLIEDLGDALFTEVINNDPAQ--EMPLYRAAVDLLIHLHDAQTPELAR--------------------------LDPETLSEMTR----LAFSEYRYAILGDAAEDNRKRFEHRFAQILSAQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLL-----QDARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFERGLAHPALASAAEEILNALPAPAPEVLERLRA

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1427 46534 32.61 153.07 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 32.61 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.277

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: