Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKMRNLISNYELAKDLLKNWEYDEENLDEMLSYFRI-SSNAIYPFFYK-KKICFLRIAPTDEKR-KENIYGEIEFIQYLREH-DFKALCPVPSKNGEFIRIVNTKWGEYYATVFEKVAGKPVEACEYSEKMYHEYGKTMGRMHRLSSGFKPII-------------KKWTHEDVLNE--IETMLTLYHC----SNKAKIELENLRVELAKLPKNHLSYGLIHYDFELDNVFYDEKTNTCSVIDFDDGMYHWYSTDIEQFLDSVSEEKGELEAEQVKIAFFEGYQSEYPIVEETKNSIPLMRRFINLYSYVRIHHCLSDSFTSEKEWMTELRVKLTNMLKSIEETW 3F7W Chain:A ((2-271)) ---------VNSVAARVTELTGREVAAV----AERGHSHRWHLYRVELADGTPLFVKALPDDAPALDGLFRAEALGLDWLGRSFGSPVPQVAGWD--------------DRTLAMEWVDERPPT-----PEAAERFGHQLAAMHLAGAESFGATWDGYIGPLPMDNTPRSTWPEFYAEQRILPYLRRAADRGALTPGDVRLVEKVLDALDHLAGDPEPPARIHGDLWNGNVLWQDD-G-AVVIDPA-AHGGHREADLAMLALFG---------LPYLDRVRDAYNEVAPLAEGWRARIPLHQLHPLLVHVCLFG--------------------------------

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1243 -8768 -7.05 -35.50 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -7.05 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.294

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: