Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVRRLVPFNLYAKKGRF-------VMASEALATQYVSENTTIPVPRVLDVIALPSGKGKFLLMTGVKGKEYGPTGVTLYKMAENQRAVFTETLRGWFDQLRCLRPPDDHTISGFMVTGVSSYIIRH-PETVDPFASQDEFHAQRFCQPWEPYDDALRAALEKRAKMRYKICFTHGDITPHNILVDDNLRPCALVDWECAGWMPEYWEYTRALYIRERYLGWKKVFTDIFPNYEAELTIYGSIITHDMPWYYSLNVVS 3TM0 Chain:A ((37-246)) ------NENLYLKMTDSRYKGTTYDVEREKDMMLWLEG--KLPVPKVLHFERH--DGWSNLLMSEADGVLCS---EEYED--EQSPEKIIELYAECIRLFHSIDISD-----CPYTNSLDSRLAELDYLLNNDLA-DVDCENWEEDT-PFKDPRELYDFLKTE-KPEEELVFSHGDLGDSNIFVKDG-KVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGI-----------------------

General information: TITO was launched using: RESULT: Template: 3TM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 850 12673 14.91 62.74 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 14.91 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.346

(partial model without unconserved sides chains): PDB file : Tito_3TM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tm0-query.scw PDB file : Tito_Scwrl_3TM0.pdb: