Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------MSTSQGEMRGRLRSNASS---TGILSSNTYAKHTLKCSLKPEAMELIRCRHLNIQTTHVKAIELVSFNEAKNRLITKK----VIGKELFHALW----NPTNLLGRLQGHRLQ--------NPDTLVHRITEIGTWLRKYHNSSAGLT--PESTDGS-----W---------------------LVTAFAQKIR--GIRASK----LIP--------EPNLKKIEQKYSTELQKLTTPDYLSFNNAFPCQVHGDFVLYNVLVDSQQNMHI--LDFSK---------TRISS-NLEDVARFYSTLWAIAQ---------TNRTRRSLLRELPTRFLKAYGLPPL-IAETPYFRANLVYNFLAHLEAQHRGRK----TFSWNTNREMGQITRAGMKWIYQQI----------------- 1D0C Chain:A ((29-444)) GPKFPRVKNWELGSITYDTLCAQSQQDGPCTPRRCLGSLVLPRKLQTRPSPGPPPAEQLLSQARDFINQYYSSIKRSGSQAHE--ERLQEVEAEVASTGTYHLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCSSAQEMFTYICNHIKYATNRGNLRSAITVFPQRAPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVP-LEHPTLEWFAAL-------GLRWYALPAVSNMLLEIGGLEFSAAPFSGWYMSTEIGTRNLCDPHR-YNILEDVAVCMDLDTRTTSSLWKDKAAVEINLAVLHSFQLAKVTIVD----HHAATVSFMKHLDNEQKARGGCPADWAWIVPPISGSLT-----PVFHQEMVNYILSPAFRYQPDPW

General information: TITO was launched using: RESULT: Template: 1D0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1214 15182 12.51 51.99 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 12.51 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.278

(partial model without unconserved sides chains): PDB file : Tito_1D0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1d0c-query.scw PDB file : Tito_Scwrl_1D0C.pdb: