Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTGHGMAGDDWQYRKTMPQNILRPTVEAALPHVAGAEWEPLAGGRTNPVFKIN-A-DAPLVCKLSCLSGETPLFGNRALDEAAILKHLEGS-GL-APSLTDVLAVPEGEILIYSFVEGDILTQT-IPAAVRILRQVHQAEIPDNLPRL---------D--GSSAAIIQ-----KTKAILETLPVQK------AM-AISFAEPKVEL-PDTLVPRLIHGDPVPANFIQSKGKLTLIDWQCPAIGDPCEDLAIALSPAMNAVYGDGPLTPQQMKECLNAYGD-RTVSDRYRKLAPLFHWRMAAYCAWKVEQGDKDYAAGVKLELAALK 3F7W Chain:A ((2-247)) ------------------VNSVAARVTELTGR-EVAAVAERGHSHRWHLYRVELADGTPLFVKALPD--DAPALDGLFRAEALGLDWLGRSFGSPVPQVAGWDD---R-TLAMEWVDERPPTPEAAERFGHQLAAMHLAGAES-FGATWDGYIGPLPMDNTPRSTWPEFYAEQRILPYLRRAADRGALTPGDVRLVEKVLDALDHLAGDPEPPARIHGDLWNGNVLWQDDGAVVIDPA-AHGGHREADLAMLALF-----------GLPYLDRVRDAYNEVAPL------------------------------------------

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 998 11442 11.46 52.97 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 11.46 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.216

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: