Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTHVHFPWYLHSIRKQRRNTCNILQNLVSNSKTSLQNAPVLYLRFLASYRVSSETTASEAICLPTIAEIEAATELISAPDASAKVVRV---NERFAVKMGYG----IPLIEAENMKFLAANSKVPVPKVHAAFRDPDTNKTYIIMEYLPGHTLEKLLPSLDPVEKATISNLIKDAITELRKGLDPKISGP---FANQGDMNLAIIEKI---RQ---TESDQYIRLLRNMIDR-TLNSHRSVFTHGDLQPKNIMVERFGDRNVCPQFRISLLDWESAGWYPELWDFCNATIACRFK---PDWLELVPDILEQYPVEFLMMQVVYSSVFY 4H05 Chain:A ((21-248)) -----------------------------------------------------------------------------EDGASGAGVYRLRGGGRELFVKVAALGAGVGLLGEAERLVWLAE-VGIPVPRVVEGGGD--ERVAWLVTEAVPGRPASARW---PREQRLDVAVALAGLARSLHALDWERCPFDRSLAVTVPQAARAVAEGSVDLEDLDEERKGWSGERLLAELERTRPADEDLAVCHGDLCPDNVLLDPRTC------EVTGLIDVGRVGRADRHSDLALVLRELAHEEDPWFGPECSAAFLREYGRGW------------

General information: TITO was launched using: RESULT: Template: 4H05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 880 434 0.49 2.09 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 0.49 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.215

(partial model without unconserved sides chains): PDB file : Tito_4H05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4h05-query.scw PDB file : Tito_Scwrl_4H05.pdb: