Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVELAPALEEV-LRPVLGDVTVENLQRLTGGASRTTWAFSAHARSRSGTAHARSESDTAHARSESDTAQTGGAGRPLI-LRTGARDDIHASMELEAKV-QQRAAAAGAPVPH-IVNADNSPAAVGAPYLI-CDAIAGETIVRRIFRG-LDDGGRARLLE-QCATALAAI-HRADPAGIGLTS--------PDDLAGWRSRLDEIGDTTATFE-WTF-RRLADARPEPSPMR--LVHGDFRMGNL-IV-DESGLAAVLDWELTHIGEI-YEDLAWFC-IRAWRFG-----APETLGAGGLGSVETFLQAYESAADTSLDRNTFRWWLTVAT-LRWGVICRHQA-ERHLSGESPSVELAAIGRRVSE--T-EWDLLELLSGGGPR 3DXP Chain:A ((20-359)) --RFDTEALEAWMRQHVEG-FAGPLSVEQFKGQSNPTFKLVT-------------------------------PGQTYVMRAKPG------AIEREYRVMDA-LAGTDVPVAKMYALCEDESV-IGRAFYIMEF-VSGRVLWDQSLPGMSPAERTAIYDEMN---RVIAAMHTVDYQAIGLGDYGKPGNYFQRQIERWTKQYKLS-ETESIPAMDSLMDWLPQHIPQEDADLTSIVHGDYRLDNLMFHPTEPRVLAVLDWELSTLGHPMG-DFGYHCMSWHIAPGQFRGIAGLDHAALGIPDEASYRKLYEQRTGRPITGD--WNFYLAFSMFRIAGI-LQGIMKRVVDGTASSAQALDAGKRARPMAEMGWEYAKKAKQ----

General information: TITO was launched using: RESULT: Template: 3DXP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1343 -3594 -2.68 -12.18 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -2.68 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.263

(partial model without unconserved sides chains): PDB file : Tito_3DXP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3dxp-query.scw PDB file : Tito_Scwrl_3DXP.pdb: