Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVELAPALEEVLRPVLGDVTVENLQRLTGGASRTTWAFSAHARSRSGTAHARSESDTAHARSESDTAQTGGAGRPLIL-RTGARDDIHASMELEAKVQQRAAAAGAPVPHIVNADNSPAAVGAPYLICDAIAGETIVRRIFRGLDDGGRA-RLLEQCATALAAIHRADPAGIGLTSPDDL--------AGWRSRL--DEIGDTTATFEWTFRRLADARPEPS-PMRLVHGDFRMGNLIV--DESGLA--AVLDWELTHIGEIYEDLAWFCIRAWRFGAPETLGAGGLGSVETF-LQAYESAADTSLDRNTFRWWLTVATLR-----WGVICRHQAE-------RHLSGESPSVELAAIGRRVSETEWDLLELLSGGGPR------- 3CSV Chain:A ((4-333)) ------------------------------------------SREDEIRDFLATHGYADWNRTPRYQRLRSPTGAKAVLMDWSPEE--GGDTQPFVDLAQYLRNLDISAPEIYAEEHARG-----LLLIEDLGDALFTEVINN---DPAQEMPLYRAAVDLLIHLHDAQTPELARLDPETLSEMTRLAFSEYRYAILGDAAEDNRKRFEHRFAQILSAQLEGDMV--FVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLL-QDARRDVPAQVEA--------QMIDHYIQATGV--DESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFERGLAHPALASAAEEILNALPAPAPEVLERLRA

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1308 18292 13.98 64.18 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 13.98 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.277

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: