Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHSRPQISPERAAQLAAEWFEGQLDAPAELDSYADRNFLVRAPDGTKAVLKVPNVELAEDIDLQIAILKWLEARPSAPLVPRVLGPTRTIEDDAGRPTRAWMVGWIEGELWFDASPTPALREELGAALGQLARDLEDFRHPGMERHFAWNLAEANWIAEELHRFEDPARAELVCDALMQFQGRVLPRLAELPTQVIHGDANDKNLAVAEGRLVGVFDFGDACWCPAVCDLAIALAYAAMDLRLGCALPEVAAACAQVCAGYHGRRPLTALELDLLYDLLRARLAVSVTSSTRAREAEPDNAYVSASEQGAWSVLEALGAVGRRAFTATVAEACGLALAPRVPGARTGDELLAARRRRLGPSLSLSYASSGMPLYIRRGEGSWLFDEHDQAFLDCVNNV--CHVGHCHPRVVEAGAAQMARL---NTNTRYLHEGLVDYAEALCATLPAP-LEVVYLVNSGSEANELALRLARDY------TGGFDVAVLDAAYHGNTGNLVDMSPYKFDAPGGRGRREW--------VHVLPTPDPYRGAHGDDGPAYAAAL---DAADAAARGRGSRLAALFCESVLGCAGQVVPASGFLAAAYARARAAGAVCIADEVQVGFGRVGDGMWAFEAEGVVPDILTLGKPIGNGH-PLGAVVTTRAIAEALGGGR-----MEFFCTFGGNPVSAAVGAAVLAVIEDEGLVANARDTGSWLRGAFEQLAADPVLGRG--IGEVRGRGLFIGVELVEDRSTKR---PDAARASAIVAHARARGVLLSTDGPARNVIKIKPPICFADVEARILASTLARALSATMR 5LHA Chain:D ((37-455)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LVIARGDGNYITDIDGQRMLDGVGGLWNVNIGHNRASVKAAIAAQLDELAYYQTFDGIAHPRVFDLAERLTGMFAQERMARVLFSSGGSDAVETALKMARQYWIASGEPGRTRFLSLRNGYHG-----VHMGGTSV---GGNGVYHYNHGQLLAGCHLLDTPWLYRNPWDCRDPQALTAHCIRQLEEQIALLGAQTIAALIAEPVQGAGGVIVPPADYWPRLREVCDRHGILLIADEVVTGFGRSG-CMLGSRGWGVAPDILCLAKGITAGYIPLGATLFNQRIADAIENGQGFSHMIMHGYTYSGHPTACAAALAVLDIVEAEDLPGNAAKVGAQL---LEQL--QPLVERYAVVGEVRGKGLMIALDLVSDKRTRQPLDPAAGQPSRIADEARRAGVLVR---PIGNKIILSPPLTLTRDEAGLMVSALEAAFA----

General information: TITO was launched using: RESULT: Template: 5LHA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2087 -52658 -25.23 -136.77 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.65 3D Compatibility (PKB) : -25.23 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.254

(partial model without unconserved sides chains): PDB file : Tito_5LHA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5lha-query.scw PDB file : Tito_Scwrl_5LHA.pdb: