Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEDRRHPRRQLDPALIASYLAGRKIELVQLMPLGKSNTNYRLTL-ADGEVCLLRLHHPGANAERENHILR-LVREIVPVPAVLASGS-----DWSIHSFVEGIPLADAPESVHAAAEALARIASLRFTSSGWIQADG--SIAPFDFGDGKSFVESMLERAD--VRAW-------VGADTAAALRRIEAAQPPAADDEPR---LVHGDFNPTNILVHQRAVTGILDWEFSHAGSPYMDIGNLLRHTDPAQHGALEAGLVAGGLNVPADWRQQAEMVDLSSHLEFLTTQRSDAFKQRCADWIRDFVERY 3W0S Chain:A ((6-233)) -----------LTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLW----AEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIF-----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3W0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 722 -29034 -40.21 -140.26 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -40.21 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.239

(partial model without unconserved sides chains): PDB file : Tito_3W0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3w0s-query.scw PDB file : Tito_Scwrl_3W0S.pdb: