Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQAISHLLNEQLGEGDIELRNELPGGEIHAAWHLRFA-GRDLFVKCDEREL-LPIFTAEADQLELLSRSKTVSVPKVWALGSDR-DYSF----LVMDYLPARPLDA--------HNAFLLGQQIARLHQWSDQPQFGLDFDNDLSTTPQPNAWQRRWSTFFAEQRIGWQLELAAEKGLAFGNIDAIVDHVHQRLAS---HQPQPSLLHGDLWSENCALGP-N-GPYIFD-PACFWGDRECDLAMLPLH--PEQPPQIYDGYQAVSPLP-PD----FLDRQPIYQLYTLFNRAILFGGEHLVNAQRALDRVLAA 5IGI Chain:A ((10-298)) --SQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISA-AVDAGM----LIRTPTQARQKVADDVDRVRR--------EFVV----NDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQLA-----

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1174 -38613 -32.89 -148.51 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -32.89 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.296

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: