Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQAISHLLNEQLGEGDIELRNELPGGEIHAAWHLRFAGRDLFVKCDEREL-LPIFTAEADQLELLSRSKTVSVPKVWALGSDR---DYSFLVMDYLPARPLDA------------HNAFLLGQQIARLHQWSDQPQFGLDFDNDLSTTPQPNAWQRRWSTFFAEQRIGWQLELAAEKGLAFGNIDAIVDHVHQRLASHQPQPSLLHGDLWSENCALGP--NG-PYIFD-PACFWGDRECDLAMLPLH-----PEQPPQIYDGYQAVSPLPPDFLDRQPIYQLYTLFNRAILFGGEHLVNAQRALDRVLAA 3N4U Chain:A ((6-278)) -FDQVEKAIEQLYPDFTINTIEISGEGNDCIAYEI---NRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISG-FKSNL---------VLDFREKINEDNKKIKK--LLSRELK-GPQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPT--VLEKYRMKEKYWSFEKIIY-------------------

General information: TITO was launched using: RESULT: Template: 3N4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1050 -27181 -25.89 -109.60 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -25.89 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_3N4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3n4u-query.scw PDB file : Tito_Scwrl_3N4U.pdb: