Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHSRPQISPERAAQLAAEWFEGQLDAPAELDSYADRNFLVRAPDGTKAVLKVPNVELAEDIDLQIAILKWLEARPSAPLVPRVLGPTRTIEDDAGRPTRAWMVGWIEGELWFDASPTPALREELGAALGQLARDLEDFRHPGMERHFAWNLAEANWIAEELHRFEDPARAELVCDALMQFQGRVLPRLAELPTQVIHGDANDKNLAVAEGRLVGVFDFGDACWCPAVCDLAIALAYAAMDLRLGCALPEVAAACAQVCAGYHGRRPLTALELDLLYDLLRARLAVSVTSSTRAREAEPDNAYVSASEQGAWSVLEALGAVGRRAFTATVAEACGLALAPRVPGARTGDELLAARRRRLGPSLSLSYASSGMPLYIRRGEGSWLFDEHDQAFLDCV--NNVCHVGHCHPRVVEAGAAQMARLNTNTRYLH----EGLVDYAEALCATLPAPLEVVYLVNSGSEANELALRLARDYTGGFDVAVLDAAYHGNTGNLV---DMSPYKFDAPGGRGRREWVHVLPTPDPYRGAHGDDGPAYAAALDAADAAARGRGSRLAALFCESVLGCAGQVVPASGFLAAAYARARAAGAVCIADEVQVGFGRVG--DGMWAFEAEGVVPDILTLGKPIGNGHPLGAVVTTRAIAEALG-GGRMEFFCTFGGNPVSAAVGAAVLAVIEDEGLVANARDTGSWLRGAFEQLAADPVLGRGIGEVRG-RGLFIGVELVEDRSTKRPDAARASAIVAHARARGVLLSTDGPARNVIKIKPPICFADVEARILASTLARALSATMR 2ZSL Chain:A ((39-377)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFYVKRGEGAYLYTVDGARIVDLVLAYGPLILGHKHPRVLEAVEEALAR-----GWLYGAPGEAEVLLAEKILGYVKRG-GMIRFVNSGTEATMTAIRLARGYTGRDLILKFDGCYHGSHDAVLVAAG------GVPTSAGVPEAVARLTLVTPY-----ND-------VEALERVFAEYGDRIAGVIVEPVIANAGVIPPRREFLAALQRLSRESGALLILDEVVTGF-RLGLEGAQGYFNIEG---DIIVLGKIIGGGFPVGAVAGSREVMSLLTPQGKVFNAGTFNAHPITMAAGLATLKALEEEPVYSVSREAAKALEEAASEVLDRTGLPYTINRVESMMQLFIGVEEVSNAAQAR-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ZSL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1739 -19056 -10.96 -59.55 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -10.96 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.146

(partial model without unconserved sides chains): PDB file : Tito_2ZSL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2zsl-query.scw PDB file : Tito_Scwrl_2ZSL.pdb: