Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPFRSNNPITRDELLSRFFPQ-------F-HPVTTFNSGLSGGSFLIEHQG---------QRFVVRQPHDPDAP-QSAFLRQYRALSQLP-ACIAPKPHLYL-----RDWMVVDYLPGEVKTYLPDTNELAGLLYYLHQQPRFGWRI--TLLPLLELYWQQSDPAR-------------RTVGWLRMLKRLRKAREPRPLRLSPLHMDVHAGNLVHSV-------SGLKLIDWEYAGDGDIALELAAVWVEN---------------------TEQHRQLVNDYATRAKI------------YPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTG-DQQFIRLADDTWRQLLIKQ 5FUT Chain:A ((8-370)) ----TRRRAY--LWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----GAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYF---

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 967 -36117 -37.35 -138.91 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -37.35 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: