Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDESGGEARAYMLGSGIVVKTQRPHRLRPRTSLEKEAHVLSLLAGPLAGRIPRLFGYGREDTAAGPVEFLVMSRIRGCAALHAG-----LPAREALLASLADVLRSVHAADVTGLRDSGLLPADVDSAALRGRLQLGFADACDGFAAHRDRYAGAGAPERIAIAAVDALAG---LTDESPVLLHSNPGPTHVFVGD---GGLCTGLIDFGDAYASHPALDLRSWPD--PADRIVLHEAYTGGRPTT--RGFDAVWTVVMILADLNAMLHRADLAEAAGRDLALRLAQL 3TDW Chain:A ((29-281)) ---EGFRNYAILVNGDWVFRFPKSQQ--GADELNKEIQLLPLLVGCVKVNIPQYVYIGKRS---DGNPFVGYRKVQGQILGEDGMAVLPDDAKDRLALQLAEFMNELSAFPVETAISAGV-PV--------TNLKNKILLLSEAVEDQVFPL-LDESLRDYLTLRFQSYMTHPVYTRYTPRLIHGDLSPDHFLTNLNSRQTPLTGIIDFGDAAISDPDYDYVYLLEDCGELFTRQVMAYRGEVDLDTHIRKVSLFVTFDQVSYLLEGLRAR-----------------

General information: TITO was launched using: RESULT: Template: 3TDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1038 -11433 -11.01 -48.04 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -11.01 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.337

(partial model without unconserved sides chains): PDB file : Tito_3TDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tdw-query.scw PDB file : Tito_Scwrl_3TDW.pdb: