Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSDEASRFLAELGIADDSVTPITGGWASWTFATGSGRILRVARNPEIDAAHRRESRLLPELAEAVSFAVPAPTHFGVHK-GMTYMVYPRLPGRGL----------EPGDRVR-AVAPMLAELHAFPVDRAAELLGCAVTAEAWRDDYLEIGKWVEGEVLPVLDGELRDRVRREYDA--TLPDLAAISPALIHRDLGCEHILTDPETGMP--TGIIDFETATVGDPAIDYVGLLVTFGEQVTRELIAASGS---EISWRLVRFYHWLGAAHAVKYGLAERDDAIVADGIEGLRRRLPG 3TDW Chain:A ((19-293)) --------------ISIQSVESLGEGFRNYAILVNGDWVFRFPKSQQGADELNKEIQLLPLLVGCVKVNIPQYVYIGKRSDGNPFVGYRKVQGQILGEDGMAVLPDDAKDRLALQLAEFMNELSAFPVETA---ISAGVPVTNLKNKILLLSEAVEDQVFPLLDESLRDYLTLRFQSYMTHPVYTRYTPRLIHGDLSPDHFLTNLNSRQTPLTGIIDFGDAAISDPDYDYVYLLEDCGELFTRQVMAYRGEVDLDTHIRKVSLFVTFDQVSYLLEGLRARDQDWISEGLELL------

General information: TITO was launched using: RESULT: Template: 3TDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1156 -55043 -47.62 -215.01 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -47.62 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_3TDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tdw-query.scw PDB file : Tito_Scwrl_3TDW.pdb: