Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTTERAEFDADAAEAILRRACAVADLDP-DGIEILRLGDHAVFRIDG---GRVISRVGRGAD-RLASVRREVAVARWLADKEYPAARLVTDAEQPIV-IEDHPVTFWEGLADGDT----YASTAEMGVLLRRLHELEAPPFS--LPRLQPFDKVAS----RLER-AVIPESTRAFLRSMARDLEAEYGRL-EFSLPSGHLHGDFNVGNVLRDAAGHPRVIDLDGFATGPREWDLMQTAMYYDSFGWHTEAEYADFVVGYGFD----VREWAGYAVLRSVRELLMVTWLSQNAGA-------------N-P-RAADEVQKRVATLRSGGSRRDWAPF 1ZYL Chain:A ((15-320)) ----------------TIMDALFEHGIRVDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFYFAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIG-LNEYLIEPRKLFEDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRD--GPMFVDLDDARNGPAVQDLWMLLNGDKA---EQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVYYLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEP---------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1218 -5515 -4.53 -20.50 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -4.53 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.403

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: