Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -METKLGRKIGEGGCSEVYEYGTEQPVKIIK---LAKSNTSMDAMRIEYRNHRTTW-----EL----GLPVPQPFDLRVVDGRPGIVVEQIQGETL--------MQRFLRGLDQPEVSEDSPIYDDVRMTARILNALHSHSAEALPSQRESIKGALLGVESLTAPEKKEICAILDDLPVKQRLCHGDPNPGNIFVRHD----EGLLIDWMNASAGNPEADLAEYILMVRYAVLPSHLGARIIGRFHSIREMMIDAFMEEYTRLSGVTAEEVDPWMLPMAARKLSADAISDEEKGLLLHEIRVRLNAYKGAEESFRE 4WW7 Chain:A ((4-257)) EFIDKVSSYLTPDVDIAPISQGAAIVFTTTTHPYLPRAKDSHQKYIIKYRPRTLNESRLLAKLYLIPGLCVPQ---LIACDPYNGFIWLEFLGEDLPGGHGFSNLKNFLWMHDQD--PYSDLVATTLRKVGRQIGLLHWND-----------------------------------------YCHGDLTSSNIVLVRDGARWTPHLIDFGLGSVSNLVEDKGVDLYVLERAILSTH-----------------SKHAEKYNAWIMEGFEEVYREQGAKGAKKLKEVTKRFEEVRLRGRKR----------------

General information: TITO was launched using: RESULT: Template: 4WW7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 953 36297 38.09 171.21 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 38.09 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.259

(partial model without unconserved sides chains): PDB file : Tito_4WW7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ww7-query.scw PDB file : Tito_Scwrl_4WW7.pdb: