Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEAYFLQGILLMKKTLIALLSLLFSVDTLISASIIDPDVYTILEQSFPS-IPK---EQWMIQVLKGGLSGTALYKIETPT---------QQYVLRLHQSTQLSSQDEREHFALIEAAKLGIAPHVAYVSSDNRAILMEFVNHKTLTLEKAKLPENIVKIACAIRKAHEIVGHPHVGE-SLLSNAHRCHEKVLKDGL--------GTE---ENINGALELIKRYREQLSAYACPQVNVHGDLNPRNIFLTDD------NGVLLIDWAESSLEDPFYDLTYFALKHDY------------------DEVQEALLLKTYLQRQP--------------TVEETSRFTLQKKIHQAFWSLTNLYLADVQLRKHPQQKIDPQASLKSWSTYQKTYADSMEELPAQYFYELSRLNYQLAL 5FTG Chain:A ((6-373)) --------------------------------PRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSN-MLFQCSLPDTTATLGDEPRKVLLRLYG-----EAMVLESVMFAILAERSLGPKLYGIFPQ--GRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPL---ELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISS---IE--FG-------YMDYAQARFDAYFHQK----------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1068 -12924 -12.10 -46.16 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -12.10 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.436

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: