Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMNREEACRVVTVPESMSAALAGYAWARDRVGESGGAVYRLHGKPGAPDLYLKHGRHAVADAIGDEMTRLRWLAGRMPVPAVVHFDRSADEAWLLMTAVPGRTAYQLLQADPAQHDIVVDALARFLQRLHAIPTSACPFNSDHDYRLGLARSRIEAGLVDEDDFDEERAGWTARQVWDAMQALLPLAPDPVVTHGDYSLDNLLIVDGQVAGCIDAGRVGIADRYQDLAIAWNCLGEFGGSLQERFLRQYGIAAPDRRKLQFHLMLDELF
4FEV Chain:D ((5-272))IQRETSCSRPRLNSNLDADLYGYRWARDNVGQSGATIYRLYGKPNAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF


General information:
TITO was launched using:
RESULT:

Template: 4FEV.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1180 -144012 -122.04 -537.36
target 2D structure prediction score : 0.70
Monomeric hydrophicity matching model chain D : 0.89

3D Compatibility (PKB) : -122.04
2D Compatibility (Sec. Struct. Predict.) : 0.70
1D Compatibility (Hydrophobicity) : 0.89
QMean score : 0.563

(partial model without unconserved sides chains):
PDB file : Tito_4FEV.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-4fev-query.scw
PDB file : Tito_Scwrl_4FEV.pdb: