Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------MVLASNVFVSDPFNSANDPKMPFLAEALNPIKVQKYLTKALF------NQFKNIQLHAIRVIRHKLG---RRCAIAYELTV---DTQSLVIIGKARAKGLDHHSYELQRSLWQAGFSE------DSSDGISVPEPLGIIPEWQ-------MWLQKKVPGTIAT-DLLPGTEG---VAIASKIAEAAHKLHQTGIFTRRCHTMSDELNILHERLPQVAQQYPQWEKRLEQILSKCDRLGANTPELSQCGIHRDFYGDQVIVNGEHLYLIDLD------LYCQGNPALDIGNFIAHITEYSLRILGN--PNALADREAAMAERFIQFHGEAIRLGIQSYT------------TLTLVRHIYISTQISD-RSSFTEAILELCEQRLR 4O7O Chain:A ((6-454)) TLATKLPWSDWLSRQRWYAGRNRELATVKPGVVVALRHNLDLVLVDVTYTDGATERYQVLVGWD--KAAIGVADD---RTGFDALYDVAGPQFLLSLIVSSAVCGTSTGEVTFTREPDVELPFAAQPRVCDAEQSNTSVIFDRRAILKVFRRVSSGIN-PDIELNRVLTRAGNPHVARLLGAYQFGDALAYALGMVTEYEANAAEGWAMATASVRDLFAEGDLYAHEVGGDFAGESYRLGEAVASVHATLADSLGTAQATFPVDRMLARLSSTVAVVPELREYAPTIEQQFQKLAA--EAITVQRVHGDLHLGQVLRTPESWLLIDFEGEPGQPLDERRAPDSPLRDVAGVLRSFEYAAYGPLVDQATDKQLAARAREWVERNRAAFCDGYAVASGIDPRDSALLLGAYELDKAVYETGYETRHRPGWLPIPLRSIARLTA

General information: TITO was launched using: RESULT: Template: 4O7O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1347 10235 7.60 31.40 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 7.60 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_4O7O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4o7o-query.scw PDB file : Tito_Scwrl_4O7O.pdb: