Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLTSSQLNSLRSLPCFE------KVVDISLLIDGMSHTCIKVTTAL---------QVFFVKKLNSET--AETEVLSSKYAAKHGLSPRIIYHDNAWLVTDYIVGITLDKNE-LSSSQCIDTGLTLMAKLHQLVPQPNAKT--IPCLDTSESVRRLFTNPNSIPSQ-------QKLF----LADITETLTLKIGIEQKRSDVTNIICHGDINYSNIIIDET------RQSWLIDFECSHLAPVDFDLSMFIAINNI------------------SPLHIDEIVNRYMSLVPS-------------YKPNKILLTYYLLYSYFINGLWYLDNTNDAKINNHLRSLAIEQWSAFDSFVVKYHLDIPKLMPLIS 5FTG Chain:A ((10-377)) ----RRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG---EAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKM-ATFHGMKMPFNKEPKWLFGTME--------KYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSL-LESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLG-----------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1053 -20290 -19.27 -73.78 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -19.27 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.322

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: