Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLSHLVDVVRRAHPDVDLEGAGVHSGQFHDVL---IARDRVFRFPKTAGAAAELPGRVAVLTAVDAVELGVGVPVPLSEV-RDGGPH-----GFLVLSRLHGTPLERGDATS--PEVIDVVAAEFARVLRAMAGADVE--KLRLVLPV-----ADAGRWRGFAGR-VRATLFPLMSEDGRARAERELAAAVAMDHVATGLVHGDLGGENVLWQQVEELPRLTGIVDWDEAKVGDPAEDLAAV---GASYGPELVERVVALLGAGDL---WPRIRAYQGTFALQQALAGAEDGDDEELEDGLTAYR 6CD7 Chain:A ((6-297)) -TFDQVEKAIEQLYPDFTINTIEI-SGEGNDCIAYEINRDFIFKFPKHSRGSTNLFNEVNILKRIHN-----KLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKLLSRELKGPQMKKVDDFYRDILENEIYFKYY-----PCLIHNDFSSDHILFDT--EKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYRMKEKYWSFEKIIYGKEYGYMDWYEEGLNEIR

General information: TITO was launched using: RESULT: Template: 6CD7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1180 9239 7.83 34.60 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 7.83 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_6CD7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6cd7-query.scw PDB file : Tito_Scwrl_6CD7.pdb: