Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEPSLDMITDESLRTAAAIDFVCQHFSL-GELVGIGDRLKGSYNINLKITTTNGEFVVRFISSSV---SEAHLQYVSKLLAKLENEGIPVPLPVQDEKGMSFVELDGKRVQVTPF---VQGRSFLCREQQVHSSASMLRRFHHILGDEPPGPLPE-WSFYHKSSDLRERLNRLRELPTIPSKEHAEVSRVMNR-------VLHKYDKDASLLPRTIIHGD--WHFWNQKYIRNEVSCVLDFDFVRNGTRLFDIAYSIWV--------------IYLLLPNYASTFDASFLDGYGKLSDIEIYLLPAAVSRISLFFLCQSIHSA-DPAVKWKHQY--------RKQMPFLDWMEKEGKSRIRSLASAVQA-- 4OCV Chain:A ((24-378)) --------------TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSI-PLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMECGMRFLADYLEGDVYFATKYPEHNLVRSRTQIKLVREMEQR-ADETRAIVADVMETT

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1499 24959 16.65 79.74 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 16.65 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: