Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLDSPGTLKQCLISQRPLQGGLTHDNTLLICAD-GSQWVCKRSLGRH-QSGLATEANVYSLLVNTRFALPIKLVHLDIASQMLLRPYIEGTTTTSWA---QLSIEQAILLLKAIHQITATHHGKVSS------PTSLTLNPANQLRVIWRYFRSRTS---Q---RAKM--MDIYGKLSTILQNRQSLFN-TLSTFTLNHGDFHPGNLIATPAQQLVPIDWERAHFGDPAFDLALINWHGQ------DPIVNSALQARAIALYTQCPAE--QVALQKRVVCWSLVRLFNDYLYLTSNGLKVDKLAHFEETTAMLLNAAS 2QG7 Chain:D ((109-447)) -----------DSLEFQIINGG-TNILIKVKDMSKQAKYLIRLYGPKTD--NREREKKISCILYNKNIA-KKIYVFFTN---GRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSS-DFDFINYGMTRLT---

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1127 -27718 -24.59 -103.04 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain D : 0.64 3D Compatibility (PKB) : -24.59 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.367

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: