Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADRFIGSAVAVPASLDWLRATETGRIWLDKLPVLLETACEHFGLSEIGSPFSGGNVSLVVPARHDGEDVVLKLQFPDHECRHEAEALWRWNGKGAVRLLKHAPELGALLLERCRPGQFLADDTETDRIGVVAGLLRLLTIPAGDPFTRLNDEAARWRDSLDHNWMAAGKPCEQYLVDAARKALQDLCLEKEAEEVLLHQDLHGHNI-LSSERDTWLAI-------DPKPLAGDPAFALSPIVRSFEFGHSKAEARYRLDRLSEELELDRERARYWTIGQTMAWAFSSSYAERHFETVRWLLAEN 4WW7 Chain:A ((4-257)) ---EFIDKVSSYLTPDVDIAPISQGA------AIVFTTTTHPY----------------LPRAKDSHQKYIIKYRPRTLNESRLLAKLYLIPGLCVPQLIACDPYNGFIWLE------FLGE-----------------DLPGGHGFS----------NLKNFLWMHDQDPYSDLVATTLRKVGRQIGLLHWNDYC--HGDLTSSNIVLVRDGARWTPHLIDFGLGSVSNLVEDKGVDLYVLERAILSTHSKHAEKYNAWIMEGFEEVYRE--------QGAKGAKKLKEVTKRFEEVRLRGRKR

General information: TITO was launched using: RESULT: Template: 4WW7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1033 -45725 -44.26 -199.67 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -44.26 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.197

(partial model without unconserved sides chains): PDB file : Tito_4WW7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ww7-query.scw PDB file : Tito_Scwrl_4WW7.pdb: