Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTETLTKELAVTKALELPFWKAPSNAEILGGGITNFNVKITDEGRTYVVRLGEDIVEHGVMRFNELSACRAAHAAGVAPAVRYF--QKGALVQDYIPADPLVEEEIQQLKMLELIAALLGKVHRDATQLIRGPVLAFWVFHILRDYTETL--RLLDSDHISKLDDLMQKAQKLEAAVGNVHLVLCHNDLLPANIL-NENGRLWLVDWEYAGMNSPLFDLGGLATNAGLSEGLQKLLLETYFD--TPVSDELWERYNAMKCASLLRESMWSMVSEQTSKIQFDYADYTRENLLRFERAFAELQLK 4R77 Chain:B ((26-276)) ------------------------------GGMTNQNYLAKTTNKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSIKTK-FDKIAPILQTIHTSAKEL-RG---EFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLEKRL-ADLG-VDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTSQEEETFLSHYESDQTPVSHE------KIAIYKILQDTIWSLWTVYKEEQGEDFGDY---GVNRYQRA-------

General information: TITO was launched using: RESULT: Template: 4R77.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1071 11834 11.05 48.50 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : 11.05 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_4R77.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r77-query.scw PDB file : Tito_Scwrl_4R77.pdb: