Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTELNASEFRSLITSVFPELTASVFKLAAKSWDSLAVDVDDTLIFKFPRHPAAERALVKEAGVLDIVRPSLSMAVPDMHIHDGPP------IFSSHAKLDGEHLIAEDYDALGQGVRRHLADDLARFYAELHVLDADRLRQAGVGAIQPWQSPDAVRTKAL---PLLPPDIGR---FAEAVIAD---FEALPPDPHGTVFGFFDGHGWNMAFDHAQGRLNGIYDFADSGFGPLHQEFIYSNFISADLTARIVSAYEMLTGRKLDRRRIAILTGFHRLSELAELADDPAHVDLMVQNVATWAAVAAHVG 4N57 Chain:A ((33-251)) ------------IEQLYPDFTINTIQISGKGNDCIAYEINRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEV-VFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKLLSRELKGPQMKKVDDFYRDILENEIYFKYYPCLIHN------DFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFI----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4N57.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 702 -12422 -17.70 -60.89 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -17.70 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.205

(partial model without unconserved sides chains): PDB file : Tito_4N57.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4n57-query.scw PDB file : Tito_Scwrl_4N57.pdb: