Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVVAALDRLAPRLSPTATRAINARRLSGGASLETWAFDLDDGAPLILRRRTSGSALRETALPLDHEASLIRAASAVGAPAPSVVHVCAPEDDLGEAYVMRRLEGETLGRRIVRDEAFAAVRPG----LARRCGEVLARIHAVPTE--GLPELAT--SDARGELARYEAIYRELGAER-PIMEAAFRWLEAAAPSP-PERPVLVHGDFRNGNLMIHPKDG-LVGVLDWELAHLGDPAEDLGWICVNSWRF--GEWRKPVGGFGDYADLLAGYGDNIPLERVRFWQALGSLKWGVMCLMMYQSFATGADRSVERAMIGRRASETEIDLVALMEAAA 3DXP Chain:A ((41-274)) ---------------------LSVEQFKG-QSNPTFKL-VTPGQTYVMRAKPG---------AIEREYRVMDALAGTDVPVAKMYALCEDESVIGRAFYIM----EFVSGRVLWDQSLPGMSPAERTAIYDEMNRVIAAMHTVDYQAIGLGDYGKPGNYFQRQIERWTKQYKLSETESIPAMDSLMDWLPQHIPQEDADLTSIVHGDYRLDNLMFHPTEPRVLAVLDWELSTLGHPMGDFGYHC-MSWHIAPGQFRGIAG---------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3DXP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 827 -55418 -67.01 -263.90 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -67.01 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_3DXP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3dxp-query.scw PDB file : Tito_Scwrl_3DXP.pdb: