Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIQTVLSDLKLN--GKVIPVVGGDVNQTYRIKTE-HRAYFLKIHPNV-KKGFFEAEVDGLKELSAF--VRVPDTYMLGETS-EGAY----LLMEWIE-PG--K--------GDQRDLAAALANLHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQEKYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQ-GHPVFVDPA-VSYGNREQDIAMSQLFGG--FRPEFLDAYQTIFPLE-K----GWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLENF 5IGI Chain:A ((12-299)) --LYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAG--MLI--RTPTQARQKVADDV-----DRVRREFV----VND---KRLHRWQRWLDDDSSW-PDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQLAA-

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1152 -18021 -15.64 -70.39 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -15.64 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.278

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: