Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAKTHFSEHTLKDILSRYTLGELLAFEPVTTGTVQTNYFLQTTQGRFVFRYYQNR--SLESVLFESELIRYLTRCNYPCPAVLQDRNGE----HAGIYNEKPYAIFEFATGQHLDHPNEAQKRQLVQKVAELNSITRGYKPLHTP-YRLNYNVESCWELAQEATQRINTAQAREKLAWLEHTLAGL--HLPPSLPRGVCHCDFHFSNLLFTDGKFSALIDFDDANYTFLLYDLAALINPFQASFDWNTWPRFQEEENVFDFSETRTILQEYTRHRALSMDEKWHLFDVYKLSILFDCIWYFERWEGEDFYEQRKIAYLDRLGRDQFYAQIFAD 1ZYL Chain:A ((15-290)) ----------TIMDALFEHGIRVDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVA-FNGQTLLNHQGFY----FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLFEDAT-LIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPM--FVDLDDARNGPAVQDLWMLLNGDKAE------QRMQLE----------TIIEAYEEFSEFDTAEIGLIEPLRAMRLVYYLAWLMRRWADPAF------------------------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1205 -36904 -30.63 -138.22 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -30.63 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.334

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: