Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINIIVEHHFAE-LPKKINRITIGICNEVYRIALDDHDVIIRLSTSD---KYLRGSEVHIPEFKQ-LGIKVPDILASDYSQTRIPLCYQIQSKIEGQDLGVV-IESLNQTELMGLAREIATIFSKVKTLPVTTQFGVIWGGDNDVSDSWAERMKLWIQESIKHGRLTGIMNQQFEGIAKDLYRNYETYF-DSIKPTMYY-GDICSKNVMINQ----GVFNGLVDLDGLTQGDPLEALSRIKLSWYGTSHGEFYYNALIKELE---LTQSEQQLITVYALLNQLSWMCENGIQFNQNTKPIVNWEKAESDKNIYLQLASDLNNK 3TDW Chain:A ((8-279)) -YTTMIMTQFPDISIQSVESLGEGFRNYAILV---NGDWVFRFPKSQQGADELNKEIQLLPLLVGCVKVNIPQYVYIGKR--SDGNPFVGYRKVQGQILGEDGMAVLPDDAKDRLALQLAEFMNELSAFPVET-AISAG----VPVTNLKNKILLLSEAVEDQVFP--LLDESLRDYLTLRFQSYMTHPVYTRYTPRLIHGDLSPDHFLTNLNSRQTPLTGIIDFGDAAISDPDYDYVYL----LEDC-GE-LFTRQVMAYRGEVDLDTHIRKVSLFVTFDQVSYLLEGLR--------------------------------

General information: TITO was launched using: RESULT: Template: 3TDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1130 -7984 -7.07 -31.07 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -7.07 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_3TDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tdw-query.scw PDB file : Tito_Scwrl_3TDW.pdb: