Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLDKKIIKIFKDKLNEKVIKITDSSNGLAQIVKIVETKNKKYVFKNPKPGDGIMIYRENF----VCSNLKSKF-IPKVFFKTKNYLIESFLEGTR---------PERNKPEIFYYSLGKNIRKIHKVKM----TGFGELQKNGKGQYKTAKQYIDNLIIENFPKFKNIKSFDKKILKYLEKFIYENI---NCFDEKNSFLLHFDLIPDNILMQDNKLAGIIDFGDANCGPVEYDFGKLYLEISDKMFTKVLEGYGKKLEMKKIQYFVVLHLLCILPYFYKVNRKRYEKSVKLLRKFSKF 3HAM Chain:A ((36-242)) --------------------------------------NEQYVVKVPKRDSVRISQKREFELYRFLENCKLSYQIPAVVYQSDRFNIMKYIKGERITYEQYHKLSEKEK-DALAYDEATFLKELHSIEIDCSVSLFSDALVNKKDKFLQDKK----LLISILEKEQLLTD---EMLEHIET-IYENILNNAVLFKYTPCLVHNDFSANNMIFRNNRLFGVIDFGDFNVGDPDNDFLCL-LDCSTDDFGK---EFGRKV-----------------------------------------

General information: TITO was launched using: RESULT: Template: 3HAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 616 31730 51.51 170.59 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 51.51 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.219

(partial model without unconserved sides chains): PDB file : Tito_3HAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ham-query.scw PDB file : Tito_Scwrl_3HAM.pdb: