Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence HFCEVSVSYENANKQKITTATKY-----------VVPPGSVDIAVGSQM----------YAVTVDETCKRTDSISIPGRFKIDTQGK 2FFK Chain:A ((141-217)) -DCEVSIDIRCSEEEK-DSDIKTHPVLGSNISHKKVSYED---IIGSTIVDTKCVKNLEFSVRIGDMCKESSELEVKDGFKY-----

General information: TITO was launched using: RESULT: Template: 2FFK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 63 7262 115.26 129.67 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 115.26 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.177

(partial model without unconserved sides chains): PDB file : Tito_2FFK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ffk-query.scw PDB file : Tito_Scwrl_2FFK.pdb: