Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MSKIYEKYRDLITNPFDIKYKNIEIKEIISYPPAGNDVVECLCKIGKDARNVFIKIER----SKVCDFATEIKNLNYLKNNSYYSKIPNIYESGIYNNKQYIVLSKIEGERLSDILIKNDNLRKELLYKYGQELAIIHRIPISNQKIAK-QRAINSYPNKTMYTTLSKE-----------NNIKEYIKFLEDNDFKKELTTFIHGDFHYANVLWLNNDINGVIDWEYSGIGLREQDIAWACTLRPGQKFMDTMEDIQTFLKGYSEKEKFDIEKLRWCLINSYCHFYLMNENNEDYKNKLLILLRNCMEEKI 1J7L Chain:A ((2-264)) AKMR---------ISPELKKLIEKYRCVK-----DTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEG---KLPVPKVLHFERHDGWSNLLMSEADGVLCSEE-YEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEE-ELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSI--REDIGEEQYVELFFDLL--GIKPDWEKIKYYILLD--ELF-------------------------

General information: TITO was launched using: RESULT: Template: 1J7L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1108 54811 49.47 223.72 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 49.47 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.314

(partial model without unconserved sides chains): PDB file : Tito_1J7L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1j7l-query.scw PDB file : Tito_Scwrl_1J7L.pdb: