Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVSQPVRSAAEWTAGDVAGVLAQACPAARVTSIRPGPPSYSNCLWLAETVD-GRLLVRIPGRSADPEYVRATVTATHLASAAG--VPTVRYHEFVPQTPLGLPVTIQEYLPGESGTAAL-RA--------DRADLRLLASTLGDWLGTLHGVRRETFGAVTGSGERTTWTAAATDQVETALRAVPEAALPASPATIAAAFARVVAELG-PGEPASLVHGDLYLDNMLVD-NGAPAALLDFEHAHYYDRFADFGKLSELLFEWWPGAEEPFLESYRDHFPPDPADEVRLRLGVGLYALNQTAYFARWQPELVGEYRTRLGKWLAAG 5UXA Chain:A ((4-257)) ------------DIKQVIEIAKKHNLFLKEETIQFNESGLDFQAVFAQDNNGIDWVLRLPRREDVMPRTKVEKQALDLVNKYAISFQAPNWIIYT------EELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLVVHTP---EEARMSMKQRMDAVR-AKF-GVGENLWNRWQAWLNDDDMWPKKTGLIHGDVHAGHTMIDKDANVTGLIDWTEAKVTDVSHDFIFNYRAF---GEEGLEALILAYKEIGGY---------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 953 -6796 -7.13 -28.31 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -7.13 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.289

(partial model without unconserved sides chains): PDB file : Tito_5UXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxa-query.scw PDB file : Tito_Scwrl_5UXA.pdb: