Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTRRVELARLRRMAVTALGRYPLAEGRLIFISHGENTTFRHIGADGTSHLVRV-HRPSRHGRGVDSAAAVDSELAWLRALREDTDLLVPEPLAARDGASAVAVTEAGETRLCSVLRWMDGRIHEASPRP-VHLHRLGAAMASLHDQADAWTPPDGFTRIHW-DHEAFFGNVMVYGRTPAAECWTLLPPELRARFREVGARLADLIPGARDS-GLIHADLHLGNALFR-----RGAVKLIDFDDCGTGPRSYELAVALWELRGRPDYPAFRQAL-LSGYRARRDIDATHLDDFIALRQVAFD-LWYTGMAQVN-----PAFSVRLDGVHQWSLDVLDLVEAG----------------------- 3CSV Chain:A ((4-333)) --SREDEIRDFLATHGYA--------------DWNRTPRYQRLRSPTGA-KAVLMDWSPEEGGDTQPFVDLAQYLRN-------LDISAPEIYAEEHARGLLLIEDLGDAL-------FTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELARLDPETLSEMTRLAFSEYRYAILGDAAEDNRKRFEHRFAQILSAQ----------LEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDAR--RDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFERGLAHPALASAAEEILNALPAPAPEVLERLRA

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1283 -3717 -2.90 -13.13 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -2.90 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.273

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: