Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNIKESVQKLLGEEL-IVFELKGKGACNNAYYVETQ-SGKKFIIKEERPNGELKEQNSLAVEAHVIKQLSN-LNFSAPLAK-VYFISKEPNM----FGYVYIEGDLLIDAWKKLTEEERIGICQNLGVFHAELEQRVTEAMAREMGVQINTYIGLHPEVEEEYSKILTFTD-IPNGWKTLAQKTKTIFDK-TLELGVFQFLHNDAHHENIIVKD-GEITGIIDFGDSEYGEIAREFSRYIRDF-PDHAEYIIKAYEKASGHKLSRERLISNSFLSGLMDNVEDYRKGGEDCIRAEKAVSKYEDIFT 5IGH Chain:A ((12-255)) ----LYALAARHGLKLHGPLTVN-ELGLDYRIVIATVDDGRRWVLRIPRRAEV---SAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWV---VPQDSEVFAESFATALAALHAVPISAAVDA-GMLIRTPTQARQKVADDV---DRVRREFV-VNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAG-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 925 -5066 -5.48 -21.83 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -5.48 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.281

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: