Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQAISHLLNEQLGEGDIELRNELPGGEIHAAWHLRFAGRDLFVKCDEREL-LPIFTAEADQLELLSRS--KTVSVPKVWALGSDRDYSFLVMDYLPARPLDA------------HNAFLLGQQIARLHQWSDQPQFGLDFDNDLSTTPQPNAWQRRWSTFFAEQRIGWQLELAAEKGLAFGNIDAIVDHVHQRLAS---HQPQPSLLHGDLWSENCALGP-NGP-YIFD-PACFWGDRECDLAMLPLH------PEQPPQIYDGYQAVSPLPPDFLDRQPIYQLYTLFNRAILF----GGEHLVNAQRALDRVLAA 5IQC Chain:A ((13-303)) --KAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVN---NEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTD-ISECT------IDNKQNVLEEYILLRETI-----YNDLT------DIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGN--IDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTY-

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1156 -18642 -16.13 -71.70 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -16.13 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: