Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREALQKALEALFGP-----GEIAELKRLAGGASKEAWAVDYRSG------AGLHPLFLRRAGGGVIYRGTLSLEAEFRTLELARAHRVRVPRPLLYLPDLEGREAFLMERLPGESIGARVVRRPEYARARARLPEAMAEELAKIHAIPPEEAPFLPRPEGSPPWRVALEEAYRDLDALG----------EPHPALEWGLRWLREH---PPRERPPVLVHGDFRIGNLLVDEEGLLAVLDWEFAHIGDPREDLAWPLVRAWRF-GEDG--------KRLGGVGEVEPFLERYNALTGQ--DLV-------PEELFWWEVLGNVRWGLGALNQAQRHLKGEERSVELAILGRLAAEMEYEILFLLESHG 3C5I Chain:D ((16-332)) ---YIKKICLEKVPEWNHFTEDNLRVKQIL--LTNQLFEVGLKEETANNYNSIRTRVLFRIYGKHVD--ELYNTISEFEVYKTMSKYKI-APQLLNTFNG-----GRIEEWLYGDPLRIDDL-------KNPTILIGIANVLGKFHTLSRKRHLPE-HWDRTPCIFKMMEKWKNQLFKYKNIEKYNCDIHKYIKESDKFIKFMKVYSKSDNLANTIVFCHNDLQENNIINTNK-CLRLIDFEYSGFNFLATDIANFFIETSIDYSVSSYPFFEIDKKKYISYENRKLFITAYLSNYLDKVVPTPKLIDEILEAVEVQALGAHLLWGFWSIIRGYQ---------------------------------

General information: TITO was launched using: RESULT: Template: 3C5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1046 10595 10.13 39.10 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain D : 0.65 3D Compatibility (PKB) : 10.13 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_3C5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3c5i-query.scw PDB file : Tito_Scwrl_3C5I.pdb: